Microfluidics Chip
The microfluidic chip consists of a central chamber (simulating cell culture) and two lateral channels (simulating blood vessels) with three channels leading to the central chamber. Used to simulate cell culture in vitro. It can be used for 2D (two-dimensional) or 3D (three-dimensional) cell culture under chemical gradient. Because the polymer used has certain optical transparency, it can be equipped with a microscope, fluorescence microscope, and confocal microscope to assist observation.
The chip is made of biocompatible plastic, airtight, and suitable for effective gradients such as CO2 and O2. They have good optical properties, high transparency and low autofluorescence.
Easy to use: The chip is compatible with any type of optical microscope (confocal, fluorescent …), and it adopts the slide format for easy handling under the microscope.
Easy to implement: It’s reaction chamber corresponds to standard 96-well plate and can be used for automatic microscope.
Easy connection: The chip can be compatible with all microfluidic systems (injection pump, peristaltic pump, pressure control system, rocker arm system, etc.).
No drug adsorption: Unlike other PDMS devices, it is made of lipophobic thermoplastic materials, and there will be no nonspecific drug adsorption problem. Therefore, it uses fluorescence to detect immunohistochemistry.
Cell recovery option: The cell culture used by the chip can be easily recovered for further experiments.
Channel | Height | Width | Length | Total Volume |
Lateral 1 | 300 μm | 1 mm | 50 mm | 14.5 μL |
Central 2 | 300 μm | 1 mm | 39 mm | 14.5 μL |
Ashton Cox | 300 μm | 1 mm | 50 mm | 14.5 μL |
Height | Width | Length | Volume | |
Reaction chamber 2 | 300 μm | 4.6 mm | 2 mm | 3 μL |
Inlet / outlet | 8 mm | Ø = 2.3 mm | 18.4 μL | |
Reservoir | 6 mm | 3.6 mm | 7 mm | 151.2 μL |
Cell/spheroid invasion and migration, angiogenesis, metastasis, angiogenesis, chemotaxis, ischemia, cell differentiation or oxidative stress, necrosis core formation in micro devices, glucose gradient experiment.